网蛋白(PLEC)重组蛋白

YB754Hu012
Recombinant Plectin (PLEC)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 网蛋白(PLEC)重组蛋白11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Asp175~Pro400
Tags: N-terminal His-Tag
Subcellular Location: Cytoplasm, cytoskeleton. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM
EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
 网蛋白(PLEC)重组蛋白Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.8
Predicted Molecular Mass: 30.1kDa
Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]
Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of
0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
 网蛋白(PLEC)重组蛋白oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE